 Home > Biochemistry News > Plant Extracts News > Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs

Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs

 Last Update: 2021-01-18
 Source: Internet
 Author: User

Tags

rna agarose gel

gel electrophoresis ladder

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

The significance of small RNAs, being an important part of the cellular machinery, is undeniable. Several techniques have been employed for detection of specific small RNAs. Among these techniques northern hybridization is the most popular due to its universal application to RNAs of various sizes. The general procedure involves separation of denatured RNA through gel electrophoresis and subsequent transfer and fixing to a membrane. RNA on the membrane is then detected using suitable labelled oligo-probes. Here we describe a method for detection of specific small RNAs, from an RNA pool, using sequence homology based oligonucleotide
DNA
or RNA probes.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Monobenzone CAS 103-16-2 White Crystal Powder USP/BP Standard

CAS No.: 103-16-2

Cosmetics Grade

$1-1.2/KG EXW

Carob Extract

CAS No.: 84961-45-5

pharmaceutical grade/food grade

$10-13/KG FOB

Premium ingredient Panax Ginseng Extract 80%

CAS No.: 50647-08-0

Food Grade

$25-30/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.