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The formation and maintenance of high species diversity in Orchidaceae plants is influenced by the interaction of many factors, of which complex and diverse pollination patterns are important
.
The emergence of deceptive pollination, such as foodborne deception, sexual deception and mimicry of spawning grounds, or the coexistence of mimicry and reward, greatly promotes the evolution and adaptation
of orchid diversity.
In the orchid family deceptive pollination, an important evolutionary trend in pollination is the use of tiny diptera insects by orchids
.
Small diptera usually refer to small flying insects less than 5 mm in length, including the midge family (Ceratopogonidae), the family Chloropidae, and the fungus family (Ceratopogonidae).
Mycetophilidae) and others
.
Pollinated orchids by these tiny diptera insects are concentrated in the neotropical subfamily Pleurothallidinae and the Australian Diurideae
.
In the two-tailed orchid family, some species feed on fungi, or microscopic flies or mushroom mosquitoes that lay eggs on the fungus act as pollinators, in addition, orchids with gill-shaped stripes on the lip may mimic fungi attracting insects
.
However, the vast majority of species in these taxa lack pollination ecology studies, so the importance of small dipteras in pollination of orchids is underestimated
.
CorybasSalisb It belongs to the subfamily Orchidoideae, the family Diurideae, which is distributed from the Himalayas to Southeast Asia, New Guinea, New Zealand, Australia and some of its islands, with about 140 species; There are 6 species in China, which is the northern boundary
of the distribution of the genus.
The flowers of this genus are small, about 1 cm, with a small distribution and population in the wild; Its flowers resemble mushrooms, which have been speculated to be mimicry fungi and pollinated by mushroom mosquitoes, but because mushroom mosquitoes are extremely small, about 2-5 mm, flight trajectories are difficult to capture, and the frequency of flower visits is extremely low, so far, only Australian orchid enthusiast Rudie H.
Kuiter has taken photos of orchid plants pollinated by mushroom mosquitoes.
Pollination ecological studies of this genus lack empirical support, and the hypothesis of the orchid fungus hangs in the balance
.
Recently, the Wang Hong Task Force of the Kunming Institute of Botany, Chinese Academy of Sciences, carried out field work in the southern section of Gaoligong Mountain and discovered a new distribution of the genus Corybas geminigibbus in China, as well as C.
biconvex and C.
shanlinshiensis The habitat, flowering period and flower morphological characteristics of the two species were thoroughly investigated, and the observation and breeding system of pollinators in the wild was carried
out.
Generally, fungus gnats include at least 6 families (i.
e.
Sciaridae, Diadocidiidae, Ditomyiidae, Keroplatidae, Bolitophilidae).
and Mycetophilidae).
The study observed and collected Phthiniasp.
from the Mycetophilidae family in the wild (1 female), and Exechiasp (2 females and 1 male), they are pollinators of the biconvex and cedar streams, respectively, which were first reported
in the tropical and subtropical regions of the northern hemisphere.
The study combined with breeding system detection, pollinator sex identification, flower visiting behavior and flower odor analysis showed that both species were self-inbred affinity, but could not self-inbred autonomously, relying on mushroom mosquito pollination and fruiting
.
No nectar has been found in the distance between orchids, nor have mushroom mosquitoes been found to lay eggs in the flowers, but the possibility
of mimicking the egg-laying site or food-source mimicry (fungal mimicry) cannot be ruled out.
In addition, it has been observed that the flowers of the orchid provide temporary shelter for mushroom mosquitoes, which also exists habitat mimicry
.
The study suggests that the three mimicry patterns of spawning site mimicry, fungal mimicry and habitat mimicry may coexist, but more in-depth research
is needed.
The results of the study are Observations on the pollination and breeding systems of twoCorybas species (Diurideae; Orchidaceae) by fungus gnats (Mycetophilidae) in southwestern Yunnan, published China in BMC Plant Biology
, a journal specializing in botany.
Han Zhoudong is the first author of the paper, and Professor Wang Hong and Associate Professor Ren Zongxin are the co-corresponding authors
of the paper.
This research was supported
by the Category B Leading Science and Technology Project of the Chinese Academy of Sciences (XDB31000000), the Basic Resources Survey Project of the Ministry of Science and Technology of the People's Republic of China (2021FY100200), the National Natural Science Foundation of China (31971570), the Natural Science Foundation of Yunnan Province (2019FB035) and the Yunling Scholars Program (YLXL20170001).
Article link
Figure 1.
Biconvex orchids: habitats, flower morphology, and pollinators
.
A.
Habitat B.
Isodomain distribution of small brittle mushroom C.
Female mushroom mosquitoes stop on the middle sepal D .
Flower morphology E.
Female fernomycetes carrying pollen masses
Figure 2 Secretions of mushrooms and mushrooms A.
Boletus fruiting body B.
Boletus dorsal surface of the biconvex orchid group C.
mushroom mosquitoes and their eggs D.
mushroom mosquito eggs
Figure 3.
C.
fir creek: habitat, floral characteristics and pollinators A.
habitat B.
flower morphology C Pollination process D.
Female Ebacterium mosquitoes carrying pollen masses