RNA activates the trans-ssDNA cleavage activity of CRISPR/Cas14 and its application
Photo courtesy of Hainan University Recently, Hainan University researcher Wan Yi's team and Chinese Academy of Sciences academician Li Jinghong's team jointly published a paper "Target RNA activates CRISPR/Cas14a1 to trans-cut single-stranded DNA without degradation of target RNA itself" in "German Applied Chemistry"
RNA is an important genetic material of organisms, and its base mutations are closely related to the occurrence of various diseases.
Currently, the diagnosis of RNA base mutations is mostly the CRISPR/Cas13a system, and other CRISPR/Cas systems mostly rely on a combination of other methods
It is reported that the study used the activated substrate target ssDNA as a control, and designed a biochemical experiment for the target RNA to activate CRISRP-Cas14a1 trans-cleavage, including the length and deletion of the target RNA, the length of the sgRNA target fragment, and the single-base mutation of the target RNA.
The research group used the target RNA to activate Cas14a1 to trigger the trans-cleavage function to develop the ATCas-RNA analysis platform, which has a good ability to detect pathogenic microorganisms with similar 16s RNA gene sequences
Related paper information: https://doi.
https://doi.
org/10.
1002/ange.
202110384
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