Neuron: Li Yulong's laboratory develops a new GRAB fluorescent probe to detect the spatiotemporal dynamics of extracellular ATP

Purine molecules such as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine (Ado) are widely present inside and outside the cell

Purine molecules such as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine (Ado) are widely present inside and outside the cell

On December 22, 2021, Peking University's Li Yulong Laboratory published a research paper entitled "A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo" in Neuron magazine, reporting a new gene-encoded ATP probe GRABATP1.

Screenshot of the paper

Screenshot of the paper

In this work, the Li Yulong laboratory used its previously designed GRAB probe strategy (GPCR Activation-Based sensor) to develop the ATP probe GRABATP1.

In this work, the Li Yulong laboratory used its previously designed GRAB probe strategy (GPCR Activation-Based sensor) to develop the ATP probe GRABATP1.

Figure 1: The performance of ATP1.

Figure 1: The performance of ATP1.

Can the ATP1.

Figure 2: ATP1.

Figure 2: ATP1.

Can the ATP1.

Figure 3: ATP1.

Figure 3: ATP1.

When the brain is in a disease state, what kind of changes will the release of ATP show? As mentioned above, purinergic signals play an important role in immunity

In addition to the development of a highly sensitive ATP1.

In summary, in this work, the author developed a new genetically-encoded ATP fluorescent probe, which achieved high spatio-temporal resolution recording of extracellular ATP

Postdoctoral student Wu Zhaofa of the School of Life Sciences of Peking University is the first author of this article.
Professor Li Yulong and Dr.
Wu Zhaofa are the co-corresponding authors.
Undergraduate graduate He Kaikai, doctoral students Pan Sunlei, Li Bohan, Deng Fei and Wang Huan have made important contributions to the article
.
The Peking University research team and the Jing Miao team (Chen Yue, Xi Fengxue) of the Beijing Brain Science and Brain-like Research Center and the Du Jiulin team (Li Hongyu, Liu Tingting) of the Center for Excellence in Brain Science and Intelligent Technology of the Chinese Academy of Sciences (Li Hongyu, Liu Tingting), etc.
have launched a full cooperation.
The University’s State Key Laboratory of Membrane Biology, Peking University-Tsinghua Life Sciences Joint Center, National Natural Science Foundation of China , Beijing Municipal Commission of Science and Technology, Feng Foundation and Boehringer Ingelheim Postdoctoral Scholarship and other institutions and funding support
.
Natural Science Fund

Original source

Original source

A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo

A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo

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