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iNature
Cell surface proteins (CSPs), including secreted and transmembrane proteins, mediate these interactions throughout the body, from the developing embryo to senescent organ systems
.
However, there is still a lack of effective methods
for the quantitative analysis of CSP for specific cell types in vertebrate tissues.
On October 10, 2022, the Luo Liqun team from Stanford University and the Howard Hughes Medical Institute in the United States published a paper entitled "In situ cell-type-specific cell-surface proteomic profiling in mice" online in Neuron magazine The study proposes a method
for extracting the cell surface proteome by extracellular labelling (iPEEL) through extracellular in situ markers.
This is a method that utilizes proximity enzyme labeling in mice to enable spatiotemporal precision labeling and analysis
of cell surface proteomes in a cell type-specific environment of native tissues.
The study also applied the iPPEEL method to developing and mature cerebellar Purkinje cells, identifying a critical, multifaceted role
of Armh4 in dendritic morphogenesis in Purkinje cells.
between their constituent cell types.
Cell surface proteins (CSPs) mediate cell-to-cell communication
during the life processes of multicellular organisms.
From the identification of peptide hormones to neurodevelopment and the discovery of regulators of immune system function, the biochemical identification of CSPs has led to many landmark discoveries
.
Analyzing the cell surface proteome will greatly facilitate the study of
cell-cell interactions in different tissues and physiological states.
CSP mapping has been implemented in isolated mammalian cells, but lacks the techniques
for protocell-cell interactions required for tissue development and function in vivo.
Recent approaches have utilized newly engineered proximity labeling enzymes to analyze interfacial proteins or proteins in secretory pathways between two cell types, but there is no general method
to analyze mammalian cell surface proteomes in native tissues in a cell type-specific manner.
Although single-cell RNA sequencing techniques provide detailed insights into RNA expression in specific cell types obtained by isolating living tissue, the transcriptome and proteome have only some degree of correlation, making it difficult to predict protein levels
from the transcriptome.
This study proposes a method to extract the surface proteome (iPEEL) from extracellular labeling in situ cells, which maps a proximity-labeled enzyme to the cell surface of a specific cell type in transgenic mice to achieve spatiotemporal and precise analysis
of the cell surface proteome.
The researchers developed an iPEEL-like approach in Drosophila that allowed us to discover new linker molecules in the olfactory circuitry of Drosophila and demonstrated the combined role
of CSPs in executing transcription factor transmission commands.
Not only that, but the researchers also showed that iPINE can efficiently perform cell surface labeling
in different mammalian tissues.
Using iPPEEL to analyze CSPs in cerebellar Purkinje cells, it was found that different species of CSPs were selectively enriched in developing and mature cerebellar Purkinje cells, although there was a large overlap among CSPs highly enriched at the two time points
.
Furthermore, through in vivo screening of candidate regulators of dendritic morphogenesis, the key role
of Armadillo-like helical domain-containing protein 4 (Armh4) in dendritic morphogenesis in Purkinje cells was determined 。 Armh4 is a protein with no known function in the nervous system, and overexpression of Armh4 disrupts dendritic morphogenesis, an action that requires its conserved cytoplasmic domain and is enhanced
by disrupting its endocytosis.
In general, the study reports a flexible approach
to cell type-specific cell surface proteome analysis in mouse tissues with spatiotemporal precision.
These results demonstrate the potential of cell surface proteomics analysis in native tissues to identify key changes in cell surface protein repertoire and to identify new regulators of cell surface signaling events under different experimental conditions, underlining the utility of
CSP profiling in protomammalian tissues in identifying cell surface signal regulators.
Andrew Shuster and Li Jiefu, doctoral students in Luo's lab, are co-first authors
of this paper.
Original link: https://doi.
org/10.
1016/j.
neuron.
2022.
09.
025
—END—
The content is 【iNature】
