Nature Chem: a new technology, ibolt, specific location control egg

May 26, 2015 / BIOON / - a new technology can more easily locate and inhibit specific enzymes and other proteins in cells, and can turn this regulation on and off at will through light This method provides researchers with another tool to study the function of proteins in the natural cell environment This study was published online in the latest nature chemistry In this study, Jason W chin of the molecular biology laboratory of medical research, University of Cambridge, UK and his colleagues inhibited the activity of the enzyme by selectively binding small molecular inhibitors to the target protein In the process, they used a technology they had previously published, using a special gene encoding protein found in cells containing tight olefins and alkynyl unnatural amino acids Therefore, the researchers can quickly and clearly use the chemical group markers related to the reaction of the azine derivatives Chin's team has used this labeling method to develop biorthogonal living tethering (bolt), and said they have created inhibitors that can selectively regulate the activity of target enzymes, called ibolt Using ibolt, the researchers designed an enzyme to strain alkyne into the cell and then added a tetrazine inhibitor binding to the cell The selective or specific inhibition effect is caused by the alkyne tetrazine reaction and the inhibitor close to the binding site of the target protein They showed that the effect of ibolt was to specifically inhibit two closely related signaling enzymes, the kinases MEK1 and Mek2 in living mammalian cells, whose signaling pathways are present in 30% of human tumors This paper also reports the photo regulated bolt, which combines the photo switch with tetrazine inhibitor The connector changes shape with different wavelengths of light, allowing researchers to change the binding of inhibitors to achieve the purpose of randomly controlling enzyme inhibition effect At present, it seems that the limiting factor of bolt is that it can function under the condition of engineering expression of unnatural amino acids Other methods of selective inhibition of kinase require relatively less cell engineering However, there seems to be no other way to control reversible enzyme activity by the wavelength of light (BIOON Com) Biovalley original compilation and collation, welcome to reprint! Please indicate the source and the original link More information is in Biovalley information app.