Nat Microbiol: How to fight salmonella infection by targeting specific tissue proteases?

June 13, 2020 /PRNewswire/ -- When the human body is infected with a pathogenic bacteria, the body's immune system tries to destroy the intruder, one way is to initiate an inflammatory response, i.ea series of cascade reactions, including the expression of protective proteins, activation of immune cells, and the process of controlled cell death when infected cells are storedPhoto Source: Joel Selkrig/EMBL In a recent study published in the international journal Nature Microbiology, scientists from the European Molecular Biology Laboratory and others have revealed how immune cells called macrophages respond to infections with salmonella enteritis, using a method recently developed in the lab to enrich, identify and quantify all new proteins produced by cytopha cells infected with salmonella infectionThe researchers used special chemical labels to label newly produced proteins, while using mass spectrometry to separate them, which helped them analyze a complete set of cell proteins produced by macrophages, and, importantly, to determine the levels of proteins in macrophages in different infection stages and in different cell regions, the researchers said, monitoring the dynamics of these proteins and targeting them or revealing molecular mechanisms in which cells respond to pathogen infectionsIn the study, the researchers also found an unexpected finding that when cells are infected with salmonella, a family of proteins called tissue proteases (cathepsins), a protease that breaks other proteins, is usually found in small subcellular structures such as lysozymes, which were previously thought to be directly related to cell death, although researchers did not know any mechanisms and connections between the process and bacterial infectionsNow scientists have found that salmonella promotes the accumulation of newly-generated tissue proteases in the nucleus of infected cells, the protein degradation activity of tissue proteases in the nucleus of cells, or the inflammatory form of activation of procedural cell death, and this study reveals the benefits of systematically tracking changes in protein dynamics during infection, which may help clarify new pathways and mechanisms used by hosts to protect themselves from pathogen infections(BioValley Bioon.com) Original origins: Selkrig, J., Li, N., Hausmann, Aet alSpatiotemporalos spikes scares cthiscin-dependent macrophcell cell sNat Microbiol (2020)doi:10.1038/s41564-020-0736-7