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Programmed death ligand 1 (PD-L1) is normally expressed on tumor cells and immune cells such as macrophages and dendritic cells, and PD-L1 and its receptor on T cells, programmed cell death-1 (PD- 1) The interaction inhibits the tumor-killing activity of these cells
Programmed death ligand 1 (PD-L1) is normally expressed on tumor cells and immune cells such as macrophages and dendritic cells, and PD-L1 and its receptor on T cells, programmed cell death-1 (PD- 1) The interaction inhibits the tumor-killing activity of these cells
In this study, to unbiasedly and systematically screen for post-transcriptional regulators of PD-L1, we constructed a new custom RBP CRISPR mini-library containing 10 single guides per gene of 1467 known RBPs (sg)RNA, as well as positive and negative control sgRNAs, were used to quantify PD-L1 expression levels using fluorescence-activated single-cell sorting (FACS), and by incorporating CRISPR screens, DENR was identified as a regulator of PD-L1
In this study, to unbiasedly and systematically screen for post-transcriptional regulators of PD-L1, we constructed a new custom RBP CRISPR mini-library containing 10 single guides per gene of 1467 known RBPs (sg)RNA, as well as positive and negative control sgRNAs, were used to quantify PD-L1 expression levels using fluorescence-activated single-cell sorting (FACS), and by incorporating CRISPR screens, DENR was identified as a regulator of PD-L1
Figure DENR depletion reduces tumor growth and PD-L1 expression in vivo
Figure DENR depletion reduces tumor growth and PD-L1 expression in vivoIn conclusion, this study identified a RBP, DENR, that regulates PD-L1 expression to evade tumor immunity and highlights the potential of DENR as an immunotherapeutic target
In conclusion, this study identified a RBP, DENR, that regulates PD-L1 expression to evade tumor immunity and highlights the potential of DENR as an immunotherapeutic target
references:
Chen, B.
Chen, B.
, Hu, J.
, Hu, X.
et al.
DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.
Nat Commun 13, 2059 (2022).
https://doi.
org/10.
1038 /s41467-022-29754-yLeave
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