Nanoparticles can also deliver CRISPR "scissors"

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published online today in the British Journal of Science and Technology describes ways to deliver CRISPR genome editing molecules through nanoparticles rather than viruses. In the experiment, U.S. scientists used this non-viral delivery method to effectively correct the genetic mutation that causes duotrophic muscular dystrophy in mice.
CRISPR, known as the "game changer in the field of biological science", has developed into one of the hottest research tools in the field. Previous studies have shown that by intervening, CRISPR can make the genome more efficient at producing changes or mutations than previous gene editing techniques. Currently, the potential of CRISPR technology in healthcare depends on the effective delivery of three DNA editing components - Cas9 DNA shearase, guide RNA, and DNA feeds to be inserted into the genome - to specific target cells. Viruses can be used to transport these molecules, but there are security risks, limited traffic and, more importantly, reduce the editing efficiency of CRISPR components.
this time, scientists At the University of California, Berkeley, Nirne Mushi, Elena Compaq and colleagues have shown that CRISPR components can be packaged around individual nano-gold particles and then wrapped in protective polymers. The nanoparticles can effectively deliver CRISPR components to a variety of different cells, i.e., an effective non-viral CRISPR delivery mechanism appears.
Their experiments also showed that gene editing was done through esophonically mediated double-stranded DNA repair, the most accurate mechanism for repairing DNA double-stranded fractures caused by Cas9 DNA shear enzymes, and that off-target editing in muscle tissue was minimized in mice with Duchia muscular dystrophy. Du's muscular dystrophy is an X chromosomal recessive genetic disease, patients will be due to the continuous deterioration of skeletal muscle weakness or atrophy, resulting in difficulty walking, the medical community has no effective treatment. (Source: Science and Technology Daily Zhang Mengran)