Muse Gamma Universal cell quality control instrument

——Win in the "starting point", the all-round monitoring of cell status flow cytometry has been widely used in basic life science research to clinical medicine research, covering cell biology, immunology, hematology, oncology, pharmacology, microbiology, genetics and other fields in various disciplines to play an important role The traditional sheath flow system instrument has a large volume, and the complex software system needs special personnel to operate and maintain, which greatly limits the use of flow cytometer in ordinary experiments Merck's life science, adhering to the consistent innovation concept, breaks through the thinking pattern of flow R & D and brings a revolutionary and innovative generation of Muse Gamma Universal cell quality control instrument The microcapillary sampling system, which discards sheath flow, can count cells accurately and analyze cell viability Meanwhile, it has basic flow function and can be directly placed in cell room or even super clean table without special maintenance Equipped with a comprehensive pre-set experimental scheme and high-quality pre packaged reagent box, without complex operation and experimental parameter setting Built in pad touch-screen computer, guided software design, half an hour to learn flow, farewell to complex flow experiment Muse Gamma Totipotent cell quality control instrument comprehensively evaluates cell status from cell vitality, cell cycle, apoptosis and other aspects, greatly improving the accuracy of follow-up experiments At the same time, it has other applications of flow detection, such as signal pathway analysis, immunophenotype analysis, etc., which meet the needs of daily experiments, and flow detection is no longer time-consuming and laborious Application: 1 Cell absolute activity count detection: in the process of cell culture in vitro, there are always some cells in the population that die for various reasons The percentage of living cells in the total cells is what we often call cell activity The basic index of "cell quality control" is cell viability Muse Gamma Totipotent cell quality control instrument can not only count cells absolutely, but also provide accurate and objective data of cell viability, and provide accurate and objective statistical basis for cell quality control Muse Gamma The cell counting and activity detection kit adopts patent compound double fluorescent labeling dyes, which are nuclear dye and cell activity dye respectively Nuclear dye is a membrane permeable dye that distinguishes cells from cell fragments Cell viability dye is a kind of non permeable dye, which can enter dead cells with membrane damage or cells in apoptotic state to combine with nuclear DNA Through the combination of these two dyes, we can accurately distinguish the living cells, apoptotic cells, dead cells and cell fragments, and at the same time, we can quantitatively evaluate the cell viability It only needs three steps to get cell analysis results easily 2、 Cell cycle detection cell cycle refers to the whole process from the completion of one division to the completion of the next division A cell cycle consists of two stages: interphase and mitosis The interphase can be divided into three stages: DNA synthesis prophase (G1), DNA synthesis phase (s) and DNA synthesis anaphase (G2) Cell division stage: M stage The content of DNA in cells changed periodically with the process of cell cycle DNA content: interphase: G1 (pre DNA synthesis, 2C), s (DNA synthesis, 2c-4c), G2 (post DNA synthesis, 4C) Division stage: M stage (DNA 4C) Muse premixed cell cycle kit provides a simple experimental process and analysis method 3 Apoptosis detection is an important indicator of cell viability and growth, an important link in cell death process, and a common diagnostic standard for researchers in basic and clinical research Among the traditional detection methods, annexin V & dead cell is the most classical one In normal cells, phosphatidylserine (PS) was only distributed in the inner side of lipid bilayer of cell membrane In the early stage of apoptosis, PS turned from the inner side of lipid membrane to the outer side Annexin V, as a phospholipid binding protein, has a high affinity with PS it binds to the cell membrane of early apoptotic cells through PS exposed on the outside of cells So annexin V is a sensitive index to detect early apoptosis 7-AAD is a kind of nucleic acid dye It can not penetrate the whole cell membrane, but in the middle and late stage of apoptosis, 7-add can combine with the nucleus through the cell membrane The membrane of normal cells and early apoptotic cells was intact and could not bind to 7-AAD By using annexin V with 7-AAD, we can distinguish early apoptosis cells from late apoptosis cells and dead cells Based on this principle, Muse annexin V & dead cell kit is provided by Muse universal cell quality control instrument, which can be used to evaluate the apoptosis status simply and quickly 4、 In addition to the overall control of cell survival quality through the appearance characteristics and genetic changes, Muse universal cell quality control instrument for multi-color DNA damage detection has designed a series of signal pathway kits, which is convenient for researchers to conduct a comprehensive study on the inducement of changes in the process of "cell quality control" DNA damage response is a very conservative mechanism to resist DNA damage induced by external and internal factors A network composed of multiple signaling pathways is used to monitor and transmit damage signals and form an appropriate response mechanism Including cell cycle checkpoint and gene expression regulation It has been applied to DNA damage response, tumor research, genetics research and other fields Muse polychromatic DNA damage detection kit can detect the activation of ATM and H2A X in cells quickly and easily and obtain the activation percentage results by using the antibodies of phosphorylated ATM (ser1981) and H2A X (ser139) 5、 Detection of T-lymphocyte subsets by Muse human CD4 T cells can cause abnormal changes in many clinical diseases, especially in patients with impaired immune function The changes of T lymphocyte subsets are of great significance in understanding the pathogenesis of the disease, guiding the clinical treatment and controlling the occurrence and development of the disease For example, in patients with cellular immunodeficiency, CD4 + is significantly reduced, and the ratio of CD4 + / CD8 + is often inverted This index is often used to monitor HIV infected people The number and percentage of CD4 T lymphocytes were detected by Muse human CD4 T cell detection kit Simple and convenient operation can quickly obtain accurate detection results Merck life science and technology service hotline: 400 889 1988-2-2 China Technology Service Center: asianechserv@merckgroup.com