-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Some plants in the genus
Artemisia
have been used for medicinal purposes. Among them,
Artemisia iwayomogi
, commonly referred to as “Haninjin,” is one of the major medicinal materials used in traditional Korean medicine. By contrast,
Artemisia capillaris
and both
Artemisia argyi
and
Artemisia princeps
, referred to as “Injinho” and “Aeyup,” respectively, are used to treat diseases different from those for which “Haninjin” is prescribed. Therefore, the development of a reliable method to differentiate each
Artemisia
herb is necessary. We found that a random amplified polymorphic
DNA
(RAPD) method can be used to efficiently discriminate a few
Artemisia
plants from one another. To improve the reliability of RAPD amplification, we designed primer sets based on the nucleotide sequences of RAPD products to amplify a sequence-characterized amplified region (SCAR) marker of
A. iwayomogi
. In addition, we designed two other primer sets to amplify SCAR markers of “Aeyup” (
A. argyi
and
A. princeps
) along with “Injinho” (
A. capillaris
) and
Artemisia japonica
, which are also traded in Korean herbal markets. Using these three primer sets, we developed a multiplex
PCR
method concurrently not only to discriminate
A. iwayomogi
from other
Artemisia
plants, but also to identify
Artemisia
plants using a single PCR process.
