Mol Cell: New technology may capture complex interactions between early viruses and host proteins

2020 May 13 News /BioValleyBIOON / - More than 70% of the virus can cause human disease, including the initiation of human COVID-19 SARS -CoV-2 coronavirus, which can intercept cell "inside the machine" to invade the body, however, researchers are unclear how RNA viruses is the command of the host protein to self-replicateSource:CC0PublicDomainRecently, one published in the International Journal ofMolecularCellresearch report, from Van Newcastle University scientists through research and development of a new method that can identify the main interactions between the viral RNA genome and host proteins invasion of these events for virus infection and cells of the innate immune system (ie, the first host organism defense) are essentialEasier to study all potential RNA virusesmethod, called VIR-CLASP (VIRal crosslinking and solid phase purification, VIRalCross-LinkingAndSolid-phasePurification) can help researchers (rather than the RNA sequence)DrManuelAscano researchers said that in the early stages of infection the virus - the number of complex interactions between the host is considered relatively small, but this study we revealed a wealth of interactions that can show the whole process sexarticle, researchers can use this new approach the discovery of new molecular targets for viral replication, thereby using these targets to develop new therapies for treating viral infections; first use by researchers unique analogs labeled RNA viruses to infect host cells, followed by the use of special light wavelength irradiated cells, to promote cross-linking is formed between the instantaneous viral genome analogs labeled with a protein from the host cell capable of interacting with the viral genome, which can be help researchers to capture events during early infection, and these events earlier in the research process is not availableFinally researchers said, we crosslinked RNA binding proteins were purified using a protein and identify a set of techniques, and then following the use of technology VIR-CLASP mass spectrometry, researchers will be able to identify a variety of particular proteins capable of binding directly chikungunya virus or influenza virus(Biovalley Bioon.com) Original source: ByungilKim, SarahArcos, KatherineRothamel, etal DiscoveryofWidespreadHostProteinInteractionswiththePre-replicatedGenomeofCHIKVUsingVIR-CLASP ,
MolecularCell (2020) .DOI: 10.1016 / j.molcel.2020.04.013