Merck millipore biopharmaceutical upstream Technology Asia Pacific Tour: from product to complete solution supply

In the upstream process of biopharmaceutical, large-scale and safe production of cell culture is the core issue of general concern in the industry The upstream process determines the efficiency of the production process and product quality As a biopharmaceutical solution provider, Merck millipore has rich industry experience Merck millipore has long been committed to sharing cutting-edge technologies and innovative solutions In the past few weeks, Merck millipore has organized a series of technical exchanges in Mumbai, Taipei, Tokyo, Seoul, Shanghai and other places in response to the possible problems faced by the upstream process customers of biopharmaceuticals The experts at the meeting provided new ideas and comprehensive solutions for solving the problems from the aspects of cell, culture medium quality control, culture process, reactor, technical service, etc It is also the first time that the Asia Pacific region has covered all aspects of upstream technology exchanges On May 28, as the last stop of this Asia Pacific region tour speech, nearly 150 experts in the field of biopharmaceutical R & D and production at home and abroad gathered in Shanghai to discuss the challenges and solutions in the upstream process of biopharmaceutical First of all, Mr Chen Wen, head of upstream products of Merck millipore Asia Pacific region, delivered a welcome speech Biopharmaceutical Enterprises ushered in an era of opportunities and challenges This lecture officially kicked off Lin Longfei, product manager of Merck millipore, then Mr Lin Longfei, product manager of Merck millipore, briefly introduced the upstream process of Merck millipore biopharmaceutical Merck millipore focuses on life science, laboratory solutions and process solutions, providing various product consumables and complete technical services from cell line construction to clinical product production In addition, Merck millipore can provide corresponding regulatory support in accordance with GMP standards, such as dossier registration documents in CTD format Dr Lin also introduced that the acquisition of sigma Aldrich by Merck group has formed a good complementary advantage, which helps Merck millipore to provide more perfect technology and services in the world In the report, Dr Li Qiang of Anyuan biological Co., Ltd shared the key technologies for the construction of high expression production cell lines, including the optimization steps in the procedures of screening high-quality DNA, transfection and cell recovery Dr Li has done a lot of research on how to select host cells, identify high expression markers and stabilize cloning Dr Michael A Cunningham, Michael A Cunningham, introduced the improvement and optimization of the latest chemical limited medium cellvento TM in the upstream process, involving parameters, application scope and other aspects Cellvento TM Cho series culture medium has no animal source, and the culture process is flexible and time-saving It can optimize the flow additive, maintain the function of cells in the protein expression process, extend the production cycle, and the protein expression between different batches has good consistency and stability Li Jincai, executive director of the biopharmaceutical Department of Shanghai Pharmaceutical Development Co., Ltd., reviewed the historical process of the increase of cell expression in the production process in the past 30 years Dr Li Jincai, executive director of the biopharmaceutical Department of Shanghai Pharmaceutical Development Co., Ltd., introduced the technical experience from cell line construction, medium selection to cell expression At the same time, he also proposed that the consistency with the original drugs should be the first principle in the production of generic drugs, or even at the expense of production Andrew Clutterbuck, an expert of Merck millipore, introduced the challenges in the process of cell culture amplification in a three to 2000 liter disposable reactor Different technological parameters will affect the behavior of culture or the formation of products when the bioreactor is transformed from shaker In the whole process of amplification, it is very important to create the same cell culture environment In addition, two cases of Vero cell culture using 3L Mobius ® bioreactor and perfusion of monoclonal antibody production further illustrate the technical details in the amplification process At the same time, the expected new 2000L Mobius ® bioreactor also appeared in this lecture The modular hardware design is equipped with automatic control device, which can meet the user's process requirements to the greatest extent and accelerate the productivity of cell production enterprises S é Bastien Ribault, an expert at the French Biotechnology Center of Merck millipore Finally, s é Bastien, the French Biotechnology Center of Merck millipore Dr Ribault believes that it is the greatest challenge for Biopharmaceutical Enterprises to use limited resources and complete the process of DNA research to clinical application in as little time as possible He mainly introduces the definition and optimization of upstream process In order to clarify, Dr s é Bastien Ribault shared the case of Fc fusion protein production with the professionals on site, and analyzed and compared the importance of cell, culture medium, supplement and culture conditions in the process In addition, in view of the professional problems raised by people from different pharmaceutical enterprises on the site, experts also patiently explained and shared industry experience and corresponding solutions