 Home > Biochemistry News > Biotechnology News > Mechanisms of spindle pole organization and instability in human oocytes

Mechanisms of spindle pole organization and instability in human oocytes

 Last Update: 2022-03-08
 Source: Internet
 Author: User

Missing movements in human meiosis

Chromosomal errors in human eggs are a major cause of miscarriage and infertility

These errors are caused by chromosomal mismatches during oocyte maturation into eggs

Aneuploidy of human eggs is a major cause of abnormal embryonic development, leading to pregnancy loss and genetic disorders such as Down syndrome

We therefore set out to investigate how the spindle poles are organized in the absence of centrosomes and why the spindle is unstable in human oocytes

We found that the microtubule cross-linking protein NUMA (mitogen) localizes to the minus end of microtubules, where it recruits the molecular motor dynein for spindle pole focusing

We therefore asked whether spindle instability is a common feature of mammalian oocytes that use NUMA for spindle pole organization

Mammalian oocyte spindle and oocyte spindle loss are increased, resulting in oocyte spindle instability

In somatic cells, two centrosomes serve as the main MTOCs, facilitating the assembly of the bipolar spindle

Stabilize the poles and spindle

Thus, our data also reveal a potential approach to improve the fidelity of spindle assembly and chromosome segregation in human oocytes

Mammalian oocyte spindle instability

Deletion of the molecular motor KIFC1 in aMTOC-free mouse oocytes (left) and bovine oocytes (middle) results in missegregation of multipolar spindles (grey) and chromosomes (magenta), reproducing human oocytes (right) spindle instability, as shown in these immunofluorescence images

In contrast, introduction of exogenous KIFC1 stabilized spindles in human oocytes that are naturally deficient in KIFC1

Summary

Human oocytes are prone to form extremely unstable meiotic spindles, which are favorable for egg cell aneuploidy

The root cause of spindle instability is not known

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

L-Ascorbate-2-Monophosphate Feed Grade

CAS No.: 109620-90-8

Feed Grade

Potassium Sorbate from reliable manufacturer

CAS No.: 590-00-1

Food Grade

Doxycycline Hydrochloride

CAS No.: 10592-13-9

Pharmaceutical Grade

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.