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Mapping the Phytoplasma Chromosome

 Last Update: 2020-11-14
 Source: Internet
 Author: User

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separation methods

dna restriction enzymes

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Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large
DNA
fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence dor�e phytoplasma genome map as an example.

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