Mammalian Cells
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Last Update: 2020-11-10
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Source: Internet
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Author: User
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Agrobacterium
most likely can transform virtually all known plant species, and experimental protocols for
Agrobacterium
-mediated genetic transformation of yet more plant species, ecotypes, and cultivars are published almost on a daily basis. Interestingly, the
Agrobacterium
host range is not limited to the plant kingdom, and it has been shown to transform many species of fungi and even prokaryotes. The ability of
Agrobacterium
to genetically transform HeLa cells further widens the range of potential hosts of
Agrobacterium
to include humans and perhaps other animal species. Furthermore, because mammalian cells significantly differ from plant cells, they provide a useful experimental system for identification and functional characterization of plant-specific factors involved in the transformation process. Here, we present basic procedures for transfection and
Agrobacterium
-mediated genetic transformation of mammalian cells. We also demonstrate the use of mammalian cells for studies of the cellular components of the genetic transformation pathway.
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