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Recently, the genome editing team of the Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry, cooperated with Peking University and other units to discover new strategies and synergies, and made new breakthroughs in the research and development of plant-guided editing technology.
In a previous study, the team found that introducing 2 additional synonymous mismatch bases into the reverse transcription (RT) template of an ALS gene target increased editing efficiency by a factor of seven
Zhao Jiuran, a researcher at the Beijing Academy of Agriculture and Forestry, said that this study not only found for the first time that the introduction of synonymous mismatched bases can significantly improve the efficiency of plant-guided editing, but also found that N-terminal fusion reverse transcriptase is more conducive to the reverse transcription process of plants than C-terminal fusion.
Xu Wen and Yang Yongxing of Beijing Academy of Agriculture and Forestry are the co-first authors of this paper, researchers Yang Jinxiao and Zhao Jiuran are the corresponding authors, the team of Professor Zhang Bo and Zhang Chuanmao of Peking University, and the team of Associate Professor Xiao Qianlin of Southwest University participated in this work
