Main functions and functions of pharmaceutical grade turpentine

 Main functions and functions of pharmaceutical grade turpentine Songjie YouTURPENTINE OIL This product is the oleoresin exuded from several Pinaceae pine plants, and the volatile oil extracted by distillation or other methods
.

【Properties】This product is colorless to slightly yellow clear liquid; odor is specific
.

Long-term storage or exposure to the air, the odor will gradually increase, and the color will gradually turn yellow
.

This product is flammable and produces thick smoke when burning
.

This product is easily soluble in ethanol, freely miscible with or glacial acetic acid, and insoluble in water
.

The relative density should be 0.
850 to 0.
870 (general rule 0601)
.

Distillation range Take this product and measure it according to the method for determination of distillation range (General Rule 0611).
The amount of distillation at 154～165℃ shall not be less than 90.
0% (ml/ml)
.

The refractive index should be 1.
466 to 1.
477 (general rule 0622)
.

　　【Identification】(1) Take 1g of this product, add 5ml of petroleum ether (30～60℃), shake well, and use it as the test solution
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Another 1 g of turpentine control extract was taken, and the control extract solution was prepared by the same method
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Then take the α-pinene reference substance, add petroleum ether (30～60℃) to make a solution containing 40mg per 1ml, as the reference substance solution
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According to the thin-layer chromatography (general rule 0502) test, draw 1-5 μl of each of the above three solutions, and place them on the same silica gel G thin-layer plate respectively.
Use cyclohexane-acetone (9:1) as the developing solvent to develop and take out , air dry, sprayed with 5% vanillin sulfuric acid solution, heated at 105 ℃ until the spot color is clear
.

In the chromatogram of the test substance, there are spots of the same color at the positions corresponding to the chromatogram of the control extract and the chromatogram of the reference substance
.

　　(2) Take 50mg of this product, put it in a brown measuring bottle, add 5ml of absolute ethanol to dissolve, shake well, and use it as the test solution
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Another appropriate amount of β-pinene reference substance and camphene reference substance was added, and anhydrous ethanol was added to make a solution containing 0.
2 mg per 1 ml, as the reference substance solution
.

According to gas chromatography (general rule 0521) to determine
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　　With 50% phenyl-50% methyl polysiloxane capillary column (column length is 30m, inner diameter is 0.
25mm, film thickness is 0.
25μm); The temperature was raised to 50°C at a rate of ℃, and then increased to 200°C at a rate of 3°C per minute; the inlet temperature was 250°C; the detector temperature was 300°C; the split injection was performed, and the split ratio was 15:1; the flow rate was 1.
0 per minute.
ml
.

The theoretical plate number should not be less than 20,000 according to the camphene peak
.

　　Draw 1 μl of the reference solution and the test solution respectively, inject them into the gas chromatograph, and record the chromatogram
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The chromatogram of the test substance should present a chromatographic peak consistent with the retention time of the reference substance chromatographic peak
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　　【Check】Insoluble matter in ethanol Take 1ml of this product, add 7ml of 90% ethanol, shake to dissolve, and the solution should be clear
.

　　The acid value should not be greater than 0.
5 (general rule 0713)
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　　【Determination of content】According to gas chromatography (General Rule 0521)
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　　Chromatographic conditions and system suitability test 50% phenyl-50% methyl polysiloxane capillary column (column length is 30m, inner diameter is 0.
25mm, film thickness is 0.
25μm); the column temperature is programmed temperature: the initial temperature is 50°C, hold for 4 minutes, ramp up to 150°C at a rate of 20°C per minute
.

The theoretical plate number should not be less than 8000 calculated according to the α-pinene peak
.

　　Determination of correction factor Take an appropriate amount of n-butanol, accurately weigh it, and add absolute ethanol to make a solution containing 10 mg per 1 ml, as the internal standard solution
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In addition, about 70 mg of α-pinene reference substance was taken, accurately weighed, placed in a 10 ml brown volumetric flask, dissolved in absolute ethanol and diluted to the mark, shaken well, accurately measure 1 ml, put it in a 10 ml volumetric flask, and accurately add the internal standard Solution 1ml, add absolute ethanol to the mark, shake well, pipette 1μl into the gas chromatograph, and calculate the correction factor
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　　Determination method Take about 0.
25g of this product, accurately weigh it, put it in a 25ml brown measuring bottle, dissolve it with absolute ethanol and dilute it to the mark, and shake well
.

Precisely measure 1ml, put it in a 10ml brown volumetric flask, accurately add 1ml of the internal standard solution, dilute to the mark with absolute ethanol, shake well, draw 1μl and inject it into a gas chromatograph for measurement
.

　　This product contains α-pinene (C10H16) not less than 80.
0%
.

　　【Storage】shade, seal, store in a cool place
.