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LT-New Breathable Packaging Material Microbial Barrier Grading Tester Customers need to bring their own preparation materials in the following configurations (customer-supplied): 1 WEI Bacillus coagus (ATCC 9372) Bud Holding Water Suspension 2 Large Casein Digested Agar (SCDA).
3 Sterile Nitrocellulose Membrane with a diameter of 47 mm or 50 mm depending on the filtration unit, pore size of 0.
45 μm4 sterile filtration unit.
5 sprayer.
6 sterile forceps.
7 incubators, 30 °C to 35 °C.
8 disc cutter, φ47 mm or φ50 mm, depending on filtration unit.
9 Fungal gloves.
10 Sterile syringe, 3 mL, with needle or micropipette.
11 Sterile pipette, 0.
1mL, 1mL, 10 mL and 25 mL
.
12 homogenizer with 300 mL mushroom homogenizer bottle
.
13 Scroll mixer.
14 Vacuum pump with air filtration.
15 Calibrated timer.
16 Calibrated flow meter, one range from 5 L/min to 30 L/min, six ranges 1.
0 L/min~ 5.
0 L/min.
17 sterile culture plate.
18 sterile water, 100 mL for washing and 9.
9 mL for dilution
.
19 Hoses and lines 20 Perforated rubber stoppers 21 Trap bottles 22 Calibrated vacuum gauge23 Compressed air source with air filter 24 Biosafety cabinet 25 Chlorine bleach powder or suitable spore killer
