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| Low-cost single-molecule peptide sequencing is expected |
The study of protein sequence is of great significance for predicting protein structure, detecting diseases and developing protein drugs
.
However, currently available protein sequencing methods include mass spectrometry sequencing and Edman degradation methods, which have problems such as complex operation process, large sample demand, high purity requirements, long time-consuming, and low throughput
Recently, the team of Bai Jingwei, a researcher at the School of Pharmacy of Tsinghua University, used helicase to control the movement of DNA, and then controlled the peptides linked to it to pass through a nanopore in a linear sequence of amino acids, and detected its amino acid sequence through changes in electrical signals
.
Related research published November 12 in the British "Journal of the Royal Society of Chemistry" ( Royal Society of Chemistry )
" Royal Society of Chemistry " ( Royal Society of Chemistry )
The scientific research team also observed a very interesting phenomenon of oscillating current.
When there are positively charged amino acid residues, this kind of oscillating current will disappear, which also illustrates the important effect of electric charge on the movement of peptides
.
Therefore, the scientific research team carried out a systematic controllable peptide perforation study to prove the importance of peptide stretching in the detection area
In addition, the scientific research team has also found that the dimer of helicase can realize repeated sequencing of the same peptide, which lays the foundation for further improving the accuracy of peptide sequencing
.
In the future, in order to successfully distinguish different amino acids, on the one hand, the resolution of nanopores can be improved on the basis of this research , and on the other hand, the polypeptide chain can be stretched by electroosmotic flow.
These improvements will be low-cost single-molecule peptides.
The development of sequencing technology has paved the way for scientific discovery and clinical applications
.
(Source: China Science News, Chunlei Shen)On the basis of this research
Related paper information: http://doi.
http://doi.
org/10.
1039/D1SC04342K
