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According to the latest news from the Chinese Academy of Agricultural Sciences, led by the Institute of Tea research and the Shenzhen Agricultural Genomics Research Institute, in cooperation with the Kunming Institute of Zoology of the Chinese Academy of Sciences and the Tea Research Institute of yunnan Academy of Agricultural Sciences, the genome of the "Longjing 43" chromosome level was assembled for the first time, explaining the high quality and anti-reverse molecular nature of "Longjing 43" and mapping the evolutionary road map for the cultivation of tea trees. This research provides abundant material for tea tree genomics and breeding research, as well as tea tree genetic and evolutionary research. The findings were published online in Nature Communications.
Yang Yajun, a researcher at the Tea Institute of the Chinese Academy of Agricultural Sciences, said that tea originated in China and is popular all over the world, tea trees are widely distributed in China, rich in species resources, six teas have their own characteristics, and there is little research on the evolution of tea trees.The
research team first took the famous fine tea tree variety "Longjing 43" as the material, and used the three generations of assembly software independently developed by the research team to combine high Hi-C and other technologies to overcome the complex genome assembly problem with high genomic hybridization and high repeat sequence ratio, and completed the genome assembly of "Longjing 43" chromosome level. Longjing 43 genome size of 3.26Gb, scaffold N50 reached 143.85Mb, with good continuity, in the single base level and scaffold level have achieved high accuracy, obtained 33,556 high-quality annotated genes. The study found that "Longjing 43" and disease resistance, flavor metabolism and self-indesocision and related gene families expanded, and anti-reverse and other related genes occurred positive selection.
Based on assembled, high-quality genomes, the team resequenced 139 representative tea tree materials from different countries and regions of the world, revealing systematic relationships between tea tree populations and depicting the evolutionary history of cultivated tea trees. The study found that with the expansion of cultivated areas, the hybridity and extensive gene flow among tea tree populations increased, and the wild close-edge population of tea trees was small and medium leaf tea varieties (most of which belong to tea variants in plant classification) and large leaf tea varieties (most of which belong to Assam tea variants in plant classification). The ancestors, in the process of domestication, there are differences in the direction of choice between the two, small and medium-leaf tea (tea variant) in the long-term domestication process related to tea flavor of the terpene metabolic genes and disease-resistant genes were selected stronger than the large leaf tea (Assam variant) tea tree.
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