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Liquid liquid filtration membrane bacteria interception tester First, manufacturer: the use of 7-inch Viluntong LCD touch screen, Chinese menu display
.
Nominal specifications, set loads, print settings, testing, uplink, downlink, time, calibration
.
Menus on the LCD display controlled by keyboard and touch, onboard printing test results
.
Second, the standard: fully in line with the YYT0918-2014 liquid filtration membrane bacteria interception tester standard in the relevant provisions of the design and manufacture
.
Third, the technical indicators test items: medicinal liquid filtration membrane bacteria interception test PLC control system, good stability, good repeatability of the differential pressure sensor range: 0-1000kpa filter membrane: direct 47MM, pore diameter 0.
45UM a box of pressure sensor range: 0-500KPA gas storage tank: stainless steel production test software: the company's independent research and development control system: PLC touch screen: 7 inch color touch screen Velentong brand test group: 2 groups; Lumen components: test components, positive control components, negative control components set time: 1s ~ 60min, customer arbitrary setting Accuracy: 0.
2S pressure system is used to provide constant challenge pressure, composed of
air pump, constant pressure tank and pressure gauge.
Instrument according to the standard: the provisions of the upgrade service
.
The free warranty period of the device is 1 year
.
Iv.
List 1, Biosafety Cabinets; 2, bacterial liquid self-matching; 3.
Colony counter; 4, air compressor ordinary 0.
3MPA or above can be; 5, biochemical incubator; five, test step 1, negative control 1.
1 control before the bacterial warfare test, and control membrane with test analysis membrane and positive control analysis membrane simultaneous culture in the pressure vessel added at least 500mL of brine lactose broth or sterile saline
.
Close valves A and B and adjustable valves, open the valve to pressurize the pressure vessel to 200 kPa, slowly open adjustable valve D, and fill the liquid with negative control filter components and its downstream analytical filtration components
.
The air in each filter is discharged into a
suitable disinfectant.
When each filter assembly is filled with liquid, close its exhaust valve
.
Open adjustable valve D, and adjust the flow rate with adjustable valve C to negative control filter membrane per square centimeter effective filtration area of 2mL/min~4mL/min When all liquid filtration is completed, close valve C and adjustable valve to close the air regulator and release the pressure
in the container.
Remove this analytical filtration component, vacuum downstream for 15 s, remove all liquids, transfer the membrane from the filtration module to M plate counting agar under sterile conditions, culture at 30 °C at 2 °C, record the number of
colonies at 72 h and 7 days.
2.
Bacterial challenge test and positive control test add the desired volume of prepared bacterial suspension
(at least 500 mL per branch) to the pressure vessel.
Close Valves C and Adjustable Valves ABC and D Open Valves A and B Pressurize the pressure vessel to 200kPa and slowly open The adjustable Valve D allows the Challenge Bacteria suspension to fill the test sample filtration assembly, the positive control filtration assembly, and their downstream analytical filtration components
.
The air in each filter assembly is discharged into the
appropriate disinfectant.
When each filter assembly is filled with liquid, close its exhaust valve
.
Open adjustable valves A and B open adjustable valve D, and adjust the flow rate per square centimeter of the test filter membrane and positive control filter membrane effective filtration area of 2mL/min~4mL/min when all liquid filtration is completed, close the valve AB and adjustable valve AB132.
8 close the air conditioner and release the pressure
in the container.
Remove each branch analytical filtration component, vacuum 15s downstream, remove all liquids, transfer each membrane from the filtration module to M plate counting agar under sterile conditions, incubate at 30 °C at 2 °C, and record the number of
colonies at 72 h and 7 days.
Identify whether each colony is defective Pseudomonas or contaminated