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The Leaf Extraction and Analysis Framework Graphical User Interface (LEAF GUI) software is designed for biologists who wish to analyze the structure of vessel bundles (veins) in leaves. The software enables users to extract descriptive statistics on the dimensions and positions of leaf veins and areoles by utilizing a series of thresholding, cleaning, and segmentation algorithms applied to images of leaf veins. The resulting statistics for the dimensions of individual veins and the areoles they surround can then be used to evaluate numerous hypotheses regarding the structure and function of leaf veins.