Lang Zhaobo's research group reveals the molecular mechanism of the new RdDM pathway (2021-08-03)

Lang Zhaobo's research group reveals the molecular mechanism of the new RdDM pathway

DNA methylation is a conservative epigenetic modification, which is essential for gene expression and genome stability

In this study, researchers through genome-wide methylation sequencing and differentially methylated regions (DMRs) analysis found that the flowering regulatory gene FVE can affect the DNA methylation level of some of the regulatory sites of the RdDM pathway

In addition, although the relationship between the RdDM pathway and histone modification has been deeply studied, the existing molecular mechanisms cannot fully elucidate the regulatory relationship between histone modification and the site of action of RdDM

Through FVE immunoprecipitation and mass spectrometry (IP-MS) IP-MS experimental analysis, it was found that FVE and RDM15 protein can interact.

The above studies were published online in The Plant Journal and Nature Communications on July 27, 2021 and June 7, 2021, respectively, entitled "MSI4/FVE is required for accumulation of 24-nt siRNAs and DNA methylation at a subset of target Regions of RNA-directed DNA methylation" and "A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation" and research papers

Researcher Lang Zhaobo from the Center for Excellence in Molecular Plant Science of the Chinese Academy of Sciences is the corresponding author of the paper.

Paper link:

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FVE interacts with RDM15 to regulate DNA methylation and siRNA accumulation at relevant sites in the RdDM pathway

The picture shows that FVE's DMR (A), siRNA differential expression region (B), and chromatin binding site (C) analysis experiments confirmed that FVE is involved in DNA methylation and 24-nt siRNA at downstream regulatory sites in the RdDM pathway Cumulative level