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Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

 Last Update: 2020-11-18
 Source: Internet
 Author: User

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The moss
Physcomitrella patens
has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the
P. patens
cell wall is similar to that of vascular plants and phylogenetic analyses of glycosyltransferase sequences from the
P. patens
genome have identified genes that putatively encode cell wall biosynthetic enzymes, providing a basis for investigating the evolution of cell wall polysaccharides and the enzymes that synthesize them. The protocols described in this chapter provide methods for targeted gene knockout in
P. patens
, from constructing vectors and maintaining cultures to transforming protoplasts and analyzing the genotypes and phenotypes of the resulting transformed lines.

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