Journal of Cell Biology: A new phenomenon that cell migration patterns regulate migratory body formation

On February 18, the research group of Jiu Yaming of the Pasteur Institute, Shanghai, Chinese Academy of Sciences published an article online in the Journal of Cell Biology entitled " Cell migration orchestrates migrasome formation by shaping retraction fibers ", which quantitatively analyzes cell migration Two key indicators of the pattern ( persistence and velocity ) regulate the formation of migratory bodies, and it is found that cell persistence can affect the formation of migratory bodies by affecting contractile fibers

The migrasome is a recently discovered cellular structure with a vesicle-like morphology

Notably, the formation of migratory bodies is strictly dependent on cell migration

In order to clarify the effect of different migration modes on the formation of migratory bodies, the researchers first observed the random migration of single TSPAN4-GFP-labeled L929 cells by live cell imaging.

The researchers tried to screen out a molecule that is not endogenously expressed on the migratory body, but regulates cell migration behavior, and finally they turned their attention to the intermediate filament vimentin [9]

In this study, the authors found that: 1) during non-sustained migration, cells formed fewer migratory bodies due to the narrowing of the cell's tail as the cells turned, producing fewer retracting fibers; 2) in addition to the persistence of movement 3) Loss of vimentin leads to defects in cell migration persistence and migration speed, resulting in less migration than wild-type cells body

Figure 1.

　　Fan Changyuan, a doctoral student of the Pasteur Institute of Shanghai, Chinese Academy of Sciences, is the first author of the article; researcher Ji Yaming of the Pasteur Institute of Shanghai, Chinese Academy of Sciences is the corresponding author of the article;Professor Ji Baohua of Zhejiang University and researcher Li Hui of Suzhou Institute of Biomedical Engineering, Chinese Academy of Sciences provided support for the data analysis of this article; the research was also supported and helped by the donation of cells from Professor Yu Li of Tsinghua University

　　【Introduction to the laboratory】 

　　The research group of Jiu Yaming from the Pasteur Institute of Shanghai, Chinese Academy of Sciences has been focusing on the research of cytoskeleton regulation of tumor metastasis and pathogen infection, optimizing and integrating a series of advanced optical imaging technology and virus and bacterial infection experimental system.

　　Original link: https://rupress.

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