Jingdong stinking frog antibacterial peptide invented by Chengdu Institute of biology and its preparation method

Recently, Chengdu Institute of biology, Chinese Academy of Sciences "a Jingdong stinking frog antibacterial peptide and its preparation method and use" was granted an invention patent by the State Intellectual Property Office Antimicrobial peptides have the characteristics of small molecular weight, good water solubility, low antigenicity, high stability, especially are not easy to produce drug resistance, which is the best choice to replace antibiotics Amphibians have their own innate immune system, the core of which is antimicrobial peptide Amphibian skin has been recognized as a huge reservoir of bioactive peptides and biogenic amines, and its skin contains a large number of bioactive molecules, which is a natural drug resource Jingdong stinking frog belongs to Yunnan stinking frog, which belongs to amphibia Anura subfamily stinking frog It is distributed in Yunnan and Guangxi Its skin has good antibacterial activity Researchers from Chengdu Institute of biology have isolated and purified an antibacterial peptide from the skin of Odorrana Jingdong by crude extraction, gel filtration chromatography, cation exchange chromatography, reversed-phase column chromatography and reverse phase HPLC column chromatography It is a single chain polypeptides, consisting of 24 amino acids, named "Jingdong Odorrana" antibacterial peptide It has good resistance to several common resistant bacteria, such as methicillin resistant coagulase negative staphylococcus, methicillin resistant Staphylococcus aureus, extended spectrum β lactamase producing Escherichia coli, enzyme resistant β lactamase producing Escherichia coli, type I β lactamase producing Enterobacter cloacae, wild type Escherichia coli and Staphylococcus aureus, which can be used to manufacture antibacterial drugs Or antibacterial agents.