JEM: Scientists identify a special protein that promotes B-cells to resist pathogen invasion - Pdap1

, July 6, 2020 /PRNewswire/
-- Whenbacteriaenter the body, the immune system usually reacts quickly against foreign invaders, and one of the important strategies of the body's defense system is B lymphocytes, which can produce antibody targeting and neutralizing pathogens, and B cells play a key role in adaptive immunity, working together with T cells and congenital immune system components to protect the body from pathogens, allergens, and toxinsPhoto Source: Di Virgilio, MDC
In a recent study published in the international journalJournal of Theof Medicine, scientists from institutions such as max Delbruck Center for Molecular Medicine in Germany identified a special protein, Pdap1, that helps B cells resist pathogen invasion, while protecting the body from stress-induced cell deathResearcher DrDi Virgilio said successful body fluid immune responses mediated by antibodies often depend on a number of factors, and mature B-cells must modify their genes to produce antibodies that better match the surface characteristics of the pathogen, known as the key principle, which can be achieved through supermutation of the somatic cell, which is the pathogen identification part of the mutant antibody when the pathogen is activated by the pathogen and the B-cellin the body fluid immune response process, the other part of the antibody is transformed in a process called class switch recombination (CLASS switch recombination, CSR), so that B cells change the antibody subtypes they produce, unlike the same type of IgM immunoglobulin, which mainly produces in the early stages of infection, B cells produce IgG antibodies, which have a different effect, which may enhance the ability of the antibody to effectively process pathogens'Initially, we wanted to understand how category conversion recombination works, so we used crispR-Cas9 gene editing tools togenetic
modification of mouse B cells to inhibit the production of specific proteins, and with this approach we found that without Pdap1 (PDGFA-associated protein 1) protein, the category conversion recombination process is rare,' said Delgado-Benito, a researcher atThe researchers then developed a mouse model of the gene that expresses the Pdap1 protein in B cells, and after further studying it found that the protein is important for supermutation of somatic cells, without which the pathogen identification portion of the antibody rarely mutates, which reduces the likelihood of the production of highly specific mutantsin in vivo experiments, researchers found that mice that produced the Pdap1 protein in the pentavirus may have died more easily than normal, and that Pdap1 may protect B-cells from stress-induced cell death, and that when B-cells begin to grow and multiply rapidly after contact with pathogens, they experience more cellular pressure In unmodified animals, the Pdap1 protein helps B cells cope with these pressures, and without it, the procedure can be activated until the cell eventually causes the cell to die, so the Pdap1 protein not only helps B cells to consistently produce effective antibodies, but also acts as a protector (BioValleyBioon.com) original source: Ver?nica Delgado-Benito et al, PDGFA-associated protein 1 cost stuyb lymphocytes from-the-celled celled and promots antibody gene , Journal of The OSE (2020) DOI: 10.10.2084.2020.
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