JACS: new research can help early diagnosis of Alzheimer's disease

September 24, 2019 / BIOON / -- recently, researchers from Rice University have developed a new method to track the formation of soluble amyloid β polypeptide aggregates in Alzheimer's disease According to the author, angel mart í, they have developed a ruthenium based fluorescent complex that binds to and labels soluble oligomeric amyloid beta peptides This allows researchers to easily track the progress of b-amyloid peptides over time The results were published in the recent journal of the American Chemical Society (image source: www Pixabay Com) scientists have long suspected that amyloid plaques are the root cause of Alzheimer's disease, but recent studies have shown that low-level polymer molecules with repeat peptide units have a greater negative impact on the disease The fluorescent molecule takes advantage of the fluorescence anisotropy When the molecule is in the solution, it moves and rotates continuously When a molecule is small, it rotates very fast and has almost zero anisotropy However, when the probe combines with the macromolecule, its rotation speed will slow down And it has obvious polarization effect Laboratory tests have shown that oligomers can form in solution at different temperatures in a few hours Low temperature solution will slow down the formation of this process, but at physiological temperature, the formation rate of amyloid polypeptide polymer is very fast In addition, the probe was also used to observe the effect of amyloid β peptide injection on neuroblasts in real time They found that when amyloid polypeptide polymers were injected into cells, the activity of cells decreased to 60% of the original level, indicating that these oligomers were indeed toxic At present, he said, ruthenium probes can only be used in laboratories "Because there is too much light scattering, it's hard to use it in the brain However, as a laboratory tool, it will enable researchers to test the effect of other molecules on the formation rate of oligomers, so as to quickly see whether a drug can delay or prevent the formation of oligomers " Information source: probes shed new light on Alzheimer's cause information link: https:// original source: Bo Jiang, Amir aliyan, Nathan P cook, Andrea Augustine, ghibom bhak, Rodrigo Maldonado, Ashleigh D Smith McWilliams, Eric M Flores, Nicolas Mendez, Mohammad shahnaaz, Fernando J Godoy, Javier Montenegro, ines Moreno Gonzalez, angel A Mart Monitoring the formation of amino oligomers using photoluminescenceanistropy Journal of the American Chemical Society, 2019; doi: 10.1021/jacs.9b06966 original link: https://pubs.acs.org/doi/10.1021/jacs.9b06966