-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Plant cell walls are highly dynamic and chemically active components of plant cells. Cell walls consist primarily of polysaccharides, with proteins comprising approx 10% of the cell wall mass. These proteins are difficult to isolate with a high degree of purity from the complex carbohydrate matrix. This matrix traps proteins and is a source of contamination for subsequent 2-DE analysis. Mature plant tissues provide a further challenge owing to the formation of secondary walls that contain phenolic compounds. This chapter discusses protein extraction from cell walls and presents a specific method for the isolation of proteins from
Medicago sativa
stem cell walls. The method includes cell disruption by grinding, copious washes with both aqueous and organic solutions to remove cytosolic proteins and small molecule contaminants, and two different salt extractions that provide a highly enriched cell wall protein fraction from alfalfa stem cell walls. Following treatment with a commercial clean-up kit, the protein extracts yield high-quality and high-resolution 2-DE separations from which proteins can be readily identified by mass spectrometry.
