Introduction to the routine culture method of passaged cells

When the primary culture is successful, with the extension of the culture time and the continuous division of the cells, on the one hand, due to the excessive cell density, the contact between cells is inhibited by contact with each other, and the growth rate will slow down or even stop on the one hand.

① Do not rush to passaging until the cells have grown enough to cover most of the bottom wall of the flask

②In primary culture, the cells are mostly mixed growth, and the coexistence of epithelioid cells and fibroblast-like cells is common

③ The number of cells inoculated at the first passage should be larger, so that the cells can adapt to the new environment as soon as possible, which is beneficial to the survival and proliferation of cells

The so-called "passage" concept in in vitro culture technology does not equal the concept of "generation" in "parent cell" and "daughter cell" in cell biology

Passaging is the result of cell division.

Generation number and cell generation number (proliferation generation) are not the same concept

Cultured cells have the characteristics of density dependence and contact inhibition.

【SNT-002】Pancreatin (with EDTA)

【SNP-M007】Mouse Kupffer cells

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