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Introduction to the research direction and application of single-cell technology such as cell sorting and separation

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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Introduction to Single Cell Technology Research Directions Single Cell Technology and Applications

technology	application	related data
FACS micro raft	Classification and Isolation	Isolate cells for other related technical analysis Sort cells by marker of interest for grouping and possible analysis
1D tracking Fibrillar Microfluidics Traditional - Single Cell 3D	cell movement	Mobility, Persistence, and Taxi Research Cell adhesion and phenotype can be analyzed 3D parallel native environment
biological sensor	Biochemical analysis	Monitoring binding interactions associated with physiological processes
pillar microcontact printing	cell traction	Mechanical data critical to cell movement
RT-qPCR RNA FISH TIVA mass spectrometry	Genome Analysis	gene expression profiling some technical spatial information

Table 1
.
Single-cell technologies, applications, and related generative data
.

technology	advantage	shortcoming
flow cytometer	high throughput Can filter multiple markers at the same time mature technology	Requires a large population of cells, which requires cells to grow through passaging High flow rates can damage cells Not suitable for all cell types
micro raft	need a small amount Cells All cell types can be studied	Requires special facilities to spawn Limited marker screening

Table 2
.
Comparison of cell sorting and isolation techniques
.

Single Cell Analysis - Flow Cytometry and Microraft Array Technology

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