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Introduction of control classification and selection method in real-time quantitative PCR

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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1.
Controls in fluorescence quantitative PCR

1.
Positive control and negative control 2.
Amplification control 3.
Internal standard (Internal Control, IC) 3.
1 Internal reference gene 3.
2 Artificial internal standard 4.
Nucleic acid extraction control 5.
Reverse transcription control ( RT control) 6.
Blank control (Blank control) 6.
1 No template blank control 6.
2 Instrument blank control 6.
3 Fluorescent dye control 7.
Quality control (QC) 8.
Fixed value reference product 2.
Selection of control 1.
Positive control 2.
Primary screening and diagnosis

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