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Intercellular Trafficking of Macromolecules During Embryogenesis

 Last Update: 2020-11-09
 Source: Internet
 Author: User

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fluorescent cells

transition

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Plasmodesmata provide routes for communication and nutrient transfer between plant cells by interconnecting the cytoplasm of adjacent cells. A simple fluorescent tracer-loading assay was developed to monitor patterns of cell to cell transport via plasmodesmata specifically during embryogenesis. A developmental transition in plasmodesmatal size exclusion limit was found to occur at the torpedo stage of embryogenesis in
Arabidopsis
; at this time, plasmodesmata are downregulated, allowing transport of small (∼0.5 kDa) but not large (∼10 kDa) tracers. This assay system was used to screen for embryo defective mutants, designated
increased size exclusion limit of plasmodesmata
that maintain dilated plasmodesmata at the torpedo stage.

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