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Single net workbench operation management specifications First, the operation specifications of the single net workbench: 1.
Check the operating environment of the single net workbench such as ambient temperature, air quality, power supply, etc.
, and register the use information; 2.
Place the equipment required for the experiment on the net workbench table, put the sterilized materials such as culture media on the left side, and put the tools on the right side; 3.
Pull down the glass baffle, turn on the UV lamp for 30 minutes for surface sterilization; 4.
After 30 minutes, turn off the UV lamp, turn on the fan to adjust to the appropriate air volume, and turn on the lighting; 5.
Open the glass baffle to about 20cm high two hands in the operating table can be smoothly operated, with 75% alcohol wipe hands and countertop; 6.
Light the alcohol lamp, and the operation process strictly follows the aseptic operation specifications; 7.
After the operation is completed, the experimental equipment will be cleaned out of the sterile operation table, 75% alcohol will wipe the table, and the lighting, fan and baffle will be turned off; 8.
Turn on the UV lamp for 15 minutes and then turn off the UV lamp
.
Second, the management specifications of the single net workbench: 1.
Every time the net workbench is used, the experimenter should first turn on the ultraviolet lamp on the net workbench, and use the ultraviolet irradiation for 20 minutes; 2.
Turn on the power supply of the clean table, turn off the UV lamp, and spray with 75% alcohol or 0.
5% peracetic acid to wipe and disinfect the work surface; 3.
During the entire experimental process, the experimenter should operate in accordance with the sterile operating procedures; 4.
After the experiment is completed, wipe the working surface with disinfectant, turn off the working power, and re-turn on the UV lamp for 15 minutes; 5.
In case of unit failure, the animal laboratory should be notified immediately, and the personnel shall continue to use it after passing the maintenance; 6.
Experimenters should pay attention to keeping the interior clean and tidy; 7.
The filter material of the single net workbench is replaced once every 2 years, and the replacement record
is made.
