Inhibitory effect of ethyl acetate extract of Artemisia argyi on HBV

Recently, researchers from the Institute of Pharmaceutical Sciences of Zhengzhou University published a paper to observe the inhibitory effect of ethyl acetate extract of Folium Artemisiae on hepatitis B virus (HBV) in vitro The results showed that the ethyl acetate extract of Artemisia argyi had a significant inhibitory effect on HBV in vitro This article was published in the Journal of Zhengzhou University (Medical Edition), the 6th issue in 2013 HepG2.2.15 cells were treated with ethyl acetate extract of 1.6, 8.0, 40.0, 200.0 and 500.0 mg / L for 72 hours The inhibition rate of cell growth was measured by MTT method and the half toxic concentration was calculated In addition, HepG2.2.15 cells were treated with 0, 10, 20 and 40 mg / L ethyl acetate extract of Aiye and 100 mg / L lamivudine (positive control) for 3, 6 and 9 days respectively HBsAg and e antigen in the supernatant of cells were detected by enzyme-linked immunosorbent assay The number of HBV DNA plaques was measured by fluorescent quantitative PCR The inhibition rate and half inhibition concentration were calculated respectively Finally, the treatment index was calculated The half toxic concentration of ethyl acetate extract of AI ye to HepG2.2.15 cells was 104.80 mg / L, and the half inhibitory concentration to HBsAg, e antigen and HBV DNA was 1.26, 8.06 and 38.97 mg / L, respectively, in a dose-dependent manner The therapeutic indexes were HBsAg 83.2, e antigen 13 and HBV DNA 2.7, respectively.