In Vitro Propagation of Hydrangea spp.
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Last Update: 2021-01-19
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Source: Internet
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Author: User
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Hydrangea
(Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. For in vitro culture, Murashige and Skoog medium supplemented with BA (0.25 mg/L) and sucrose (30 g/L) was used. Culture conditions were 23 � 1�C of temperature, light intensity of 35 μmol/m
2
/s P.P.F.D., and 16/8 h day/night photoperiod. Following shoot proliferation, the in vitro rooting frequency was 100% on a medium containing NAA 0.5 mg/L. However, 95% direct in vivo rooting was achieved by dipping microcuttings in a 5,000 ppm K-IBA solution which were transferred afterward to a glasshouse for acclimatization. After 21 days, fully acclimatized and well-established plants were obtained, suitable for commercialization. Furthermore, leaf fragments derived from in vitro plantlets were cultured for callus induction and adventitious shoot regeneration.
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