In Planta Agrobacterium-Mediated Transformation by Vacuum Infiltration
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Last Update: 2020-11-14
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Source: Internet
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Author: User
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In planta Agrobacterium
-mediated transformation using vacuum infiltration results in transgenic
Arabidopsis thaliana
without the use of sterile conditions or plant regeneration. Plants are grown in pots, in standard potting mix.
Agrobacterium tumefaciens
, carrying an appropriate plant transformation vector, is suspended in an infiltration medium that contains, at a minimum, sucrose and the surfactant Silwet L-77. Flower buds are immersed in the suspension of
A. tumefaciens
. The application of a vacuum drives the bacteria into the intercellular air spaces. A portion of the
Agrobacterium
Ti plasmid known as the T-
DNA
region, which has been engineered to carry a selectable marker, becomes integrated into the plant genomic DNA. Plants are allowed to set seed. Seeds are germinated in selective conditions to recover transformants.
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