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Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana: Method 3

 Last Update: 2020-11-13
 Source: Internet
 Author: User

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Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism
Arabidopsis thaliana
. This chapter describes fixation, sample preparation, and immunolocalization techniques used in our laboratory. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation.

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