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The following chapter describes a
PCR
method for the identification of the raspberry root rot pathogen
Phytophthora fragariae
var.
rubi
. Furthermore, a nested PCR suitable for the detection of the pathogen in infected raspberry roots and validated against the “Duncan bait test” (EPPO Bull 35:87–91, 2005) is explained. Protocols for different
DNA
extraction methods are given which can be transferred to other fungal pathogens.