How much do you know about low adsorption centrifuge tubes?

Low adsorption centrifuge tubes are often used for protein concentration and desalting operations, buffer exchange or desalting operations for chromatographic washes and physiological gradient fragments, and recovery of biomolecules from bacterial culture supernatants or lysates of cells
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For washing and concentrating latex suspensions, centrifuge tubes can usually be used without pretreatment, but for protein sample processing, especially for thinner protein solutions, recovery is often not quantitative with membrane concentration, although PES material Nonspecific adsorption is minimized, however, some proteins can be problematic, especially when diluted.
The degree of nonspecific binding varies with individual protein structures.
Proteins containing charged or hydrophobic structures are more prone to irreversible binding to different surface
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Precautions when centrifuging low-adsorption centrifuge tubes: Avoid prolonged or high-speed centrifugation.
Although our products have a dead-end design, it can avoid irreversible adsorption caused by drying of the membrane surface caused by excessive centrifugation, and avoid excessive concentration.
The smaller the final volume, the easier it is The yield is lost due to surface adsorption.
After centrifugation, gently blow and suck the solution retained by the filter membrane several times.
If necessary, add a certain volume of buffer to rinse the membrane surface, which will help reduce the adsorption on the membrane surface and improve the recovery rate.
Avoid contact of the TIPS with the membrane surface
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Passivating the surface of the low adsorption centrifuge tube surface can reduce the loss of protein adsorption on the membrane surface.
In most cases, pretreating the column before concentrating the diluted protein solution can improve the recovery rate, because the solution can fill the membrane and expose the surface.
The method of passivation is to pre-soak the column with a high volume of passivation solution for more than one hour, rinse the column thoroughly with distilled water, and then centrifuge with distilled water once to completely remove the passivation solution that may remain on the membrane.
Be careful not to let the membrane dry after passivation.
If you want to keep it for later use, you need to add sterile distilled water to keep the membrane moist
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 Some key points of low adsorption centrifuge tubes! Our company is composed of industry professionals with industry background and rich market experience, providing products for the field of life science research and providing services for the majority of scientific research workers
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