How many proteins can more than 20000 human genes encode

The human genome encodes no less than 200000 proteins The team, led by Wei Chaochun, associate professor of life science and technology, Shanghai Jiaotong University, came to the conclusion by predicting human genome proteins based on DNA sequences and other evidences Hu Zhiqiang, a doctoral student, is the first author of a related academic paper, which has been published online in science report, a series of journals of nature Experts believe that this study improves the understanding of genome complexity and provides a more complete human reference genome annotation for biomedical research According to reports, it has been known that the number of human genes that can encode proteins is more than 20000 However, due to the variable shearing mechanism, the same gene can be expressed into many different proteins How many proteins these 20000 human genes can encode is still a mystery Although a variety of high-throughput sequencing methods have made rapid progress, many proteins are only expressed in specific tissues, at a certain stage of development or under specific conditions, so it is almost impossible to detect all human proteins by experimental methods under the current technical conditions However, the method of predicting protein sequence directly from human genome sequence can avoid this defect Wei Chaochun's research team first developed a method to predict variable splicing directly from the beginning of genomic sequence, and combined with the existing massive gene expression data, including transcriptome and proteome data, found about 30000 protein sequences that are not in the existing database Random sampling and design experiments show that about 85% of the predicted results can be verified by experiments These protein sequences increase the number of complete protein sequences in the existing database to about 90000 Compared with the existing proteins in the public database, the expression of these newly discovered proteins is more tissue-specific and less expressed, so it is more difficult to be found Based on these results, researchers predict that the human genome will encode no less than 200000 proteins Adelaide University of Australia, Vanderbilt University of the United States, China's Second Military Medical University and Shanghai Research Center for biological information technology participated in the cooperative research of the project.