How can natural pesticides be highly productive?

Natural product pesticides are highly efficient, safe, and compatible with the environment
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They have an indispensable strategic position in the prevention and control of plant diseases and insect pests and the protection of China's food security .
However, due to the generally low output, there are difficulties in the industrialization and even popularization of natural product pesticides
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How can we increase the output of natural product pesticides? The reporter learned from the Chinese Academy of Agricultural Sciences that the green target molecule design innovation team of the Institute of Plant Protection of the Academy was recently invited to systematically elaborate on the precursors in the production of Streptomyces polyketone natural pesticides in the "Current Opinion in Biotechnology".
Synthetic biology strategy for transformation of metabolic pathways and high yield of strains
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According to associate researcher Li Shanshan, the first author of the article, natural product pesticides generally refer to secondary metabolites produced by microorganisms with agricultural value.
Streptomyces is the main industrial production bacteria
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At present, all countries are focusing on the development of natural product pesticides, and their application prospects are broad
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However, due to the complex regulation mechanism of growth and metabolism of Streptomyces, it is difficult to control artificially.
In production, the yield is usually low, and the development of high-yielding bacteria is slow, especially in the fermentation industry, facing common problems such as low yield and high cost, which seriously hinder natural product pesticides.
Development and application
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Li Shanshan introduced that its yield and strain growth are inseparable from the precursor pathway.
The so-called precursor, the "raw material" of the target product of metabolism, is like the bricks used to build a building
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In the article published this time, the biosynthetic pathways of Coenzyme A precursor substances necessary for the biosynthesis of the natural polyketides of Streptomyces are systematically described, including self pathways and heterologous pathways
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The self-pathway, that is, the way through which Streptomyces synthesizes the natural product pesticides we need in its growth and metabolism; the heterologous way, that is, the production and metabolism of Streptomyces through the interference of external forces, a productive synthetic pathway
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In addition, the article also summarizes synthetic biology strategies for optimizing and adapting these metabolic pathways in the process of increasing the yield of strains
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According to reports, clarifying the synthesis mechanism of natural product pesticides and constructing efficient precursor supply channels are of great value for the healthy growth of strains and increasing the yield of target natural products.

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In actual production, this has broad guiding significance for the use of synthetic biology strategies to reconstruct more efficient natural product pesticide synthesis pathways and obtain high-yield industrial production bacteria
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The research was funded by the National Natural Science Foundation of China and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences
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From Beijing News