Having a baby too late?

Assisted reproductive technology (including human assisted reproductive technology and human sperm bank) refers to the use of medical technology and methods to artificially manipulate gametes, zygotes, and embryos to achieve the purpose of conception.

Among them, in vitro fertilization-embryo transfer technology and its various derivative technologies (In vitro fertilization, IVF) have attracted much attention in recent years (the name is too long to remember the series)

The past and present of "test-tube baby"?

The world's first test-tube baby was born in 1978, and since then a new era in the field of reproductive medicine has been opened

IVF does not really mean that babies grow up in test tubes

Classification of IVF technology

Classification of third-generation IVF

Why choose PGT-A?

Although the test tube baby technology has brought hope to infertile couples, the abnormal number of embryonic chromosomes occurs more frequently in conventional in vitro fertilization, and the incidence of chromosomal abnormalities in the embryos of older women is higher

PGT-A technology can detect chromosome aneuploidy and chromosome copy number variation on pre-implantation embryo cells, and select embryos with normal chromosome number, which can effectively improve the success rate of in vitro fertilization and help the popularization and application of assisted reproductive technology

PGT-A research method

In the past few years, PGT-A analysis was mainly carried out through fluorescence in situ hybridization (FISH) technology and comparative genomic hybridization chip (aCGH) technology

One of the difficulties in PGT-A research is to choose the appropriate single-cell whole-genome amplification technology

When conducting PGT-A research based on NGS technology, since the DNA in each embryonic cell is very small (picogram level), which is far from reaching the sample volume required for sequencing, it is necessary to perform whole-genome amplification of the DNA in a single cell first (Whole genome amplification, WGA), and this process must avoid sample loss and contamination as much as possible, and ensure the coverage and uniformity of amplification as much as possible.

Takara is committed to providing support for scientific research through excellent technology.

How does PicoPLEX technology carry out PGT-A research correctly and efficiently

• Easy to operate, avoid the loss of precious samples

The entire experimental process is "feeling like flying" (see the figure below).

• Cool "quasi-linear pre-amplification + hairpin structure" blessing to ensure the uniformity of amplification

After cell lysis, DNA is used as a template to pre-amplify using a quasi-linear amplification method, and a hairpin structure that cannot be further amplified is formed at the end of each cycle.

• Can be used for high-quality chromosome variation (CNV) analysis

After using a single blastomere biopsy sample for PicoPLEX WGA Kit expansion and labeling, hybridization with 24 sure array, the result clearly shows CNV (above)

• Case public: PicoPLEX has excellent performance when conducting PGT research

The team of Vendrell ^[2] from Sistemas Genómicos Ltd.

Expansion: Non-invasive preimplantation aneuploidy gene detection (niPGT-A)

Although embryo biopsy is considered a relatively safe method, other less invasive techniques have also received attention recently

The Jacqueline team from the University of Southern California ^[3] used whole-genome amplification of cfDNA extracted from waste embryo culture medium (SEM) to analyze the aneuploidy of embryonic chromosomes and verify the non-invasive preimplantation genetic detection of aneuploidy.

Reference information and literature sources

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