H2O2-responsive near-infrared second-region AIE nanobombs via CO-promoted low-temperature PTT

Abstract: Photothermal therapy (PTT) is a hyperthermic strategy that utilizes photothermal conversion agents (PCAs) to convert light energy into heat to ablate cancer cells

Scheme 1: (A) Schematic diagram of mPEG(CO) synthesis route; (B) schematic diagram of nanobomb composed of mPEG(CO) and PBPTV; (C) schematic diagram of nanobomb cryogenic treatment process

Research idea: The polymer carrier material mPEG(CO) was synthesized using triiron dodecacarbonyl and mercaptopolyethylene glycol (mPEG2000-SH), and further assembled with the photothermal reagent PBPTV to form a chemically triggered AIE nanobomb (PBPTV@ mPEG(CO)) (Schemes 1A and 1B)

Figure 1: (A) UV absorption (blue) and fluorescence emission (red) spectra of PBPTV@mPEG(CO) aqueous solution; (B) particle size measured by TEM and DLS; (C) PBPTV@mPEG(CO) EDS element map; (D) Heating curve recorded by FILR camera under 808 nm near-infrared laser irradiation

First, the optical characterization of the nanobomb is investigated

Figure 2: (A) Detection of CO release by changes in the absorption spectrum of reduced hemoglobin (Hb); (B) Detection of CO expression in cancer cells and normal cells by confocal fluorescence microscopy

Next, the CO release ability of the nanobombs was tested in vitro.

Figure 3: (A) Cancer cell survival in staining experiments of dead (red) and live (green) cells; (B) Western blots images of HSP70 protein under different treatments

Next, we explored whether CO could improve the therapeutic effect of hypothermic PTT

Figure 4: (A) Photothermal imaging and NIR-II fluorescence imaging of tumor-bearing mice; (B) curve of tumor volume change in mice within 18 days; (C) body weight change of mice within 18 days; (D) tumor after treatment Pictures; (E) Stained sections of tumor tissue under different treatments; (F) Fluorescence intensity under different conditions

Finally, the therapeutic effect of tumor bombs on PTT in vivo was verified

Summary: The authors developed a NIR-II AIE nanobomb that can release a large amount of CO when triggered by the tumor microenvironment (excessive H2O2), and the released CO not only inhibits tumor cells itself, but also inhibits the up-regulation of HSPs , to enhance the therapeutic effect of hypothermic PTT

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Near Infrared Zone II Small Animal In Vivo Fluorescence Imaging System - MARS 

NIR-II in vivo imaging system

High Sensitivity - Using the Princeton Instruments deep-cooled camera, the penetration depth of the living body is higher than 15mm

High resolution - customized high-resolution large aperture infrared lens, the spatial resolution is better than 3um

Fluorescence lifetime - resolution better than 5us

High-speed capture - better than 1000fps (frames per second)

Multimodal System - Scalable X-ray Irradiation, Fluorescence Lifetime, One Zone Fluorescence Imaging, In Situ Imaging Spectroscopy, CT, etc.

Microscope - Near-infrared second-region high-resolution microscopy system, compatible with imaging spectrometers

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 Hengguang Zhiying

Shanghai Hengguang Zhiying Medical Technology Co.

Compared with traditional imaging techniques based on visible wavelengths, our technology focuses on the X-ray, ultraviolet, infrared, short-wave infrared, terahertz range, and can be used for a range of subjects such as oncology, neurology, cardiovascular, pharmacokinetics, etc.

At the same time, Hengguang Zhiying also has probe research and development capabilities.
We have successfully developed more than 15 kinds of probes, which will be widely used in related research in many frontier fields of biotechnology
.