GUT: Xu Zhengping/Sheng Jinghao team found that angiogen can maintain the stability of the intestinal bacteriocytes to prevent the occurrence of enteritis.

Recently, Xu Zhengping/Sheng Jinghao Group of Zhejiang University School of Medicine, Liangyu Laboratory (Systems Medicine and Precision Diagnosis and Treatment Zhejiang Laboratory) published a research paper online entitled: Angiogenin Maintenances Gut Microbe Homeostasis by Balancing alpha-Proteobacteria and Lachnospiraceae.
The study used clinical samples, mice with angiogenin (ANG) gene loss, and a variety of enteritis models to demonstrate that intestinal secretion of ANGs maintains the micro-ecological balance of the intestines through differential regulation of the growth of alpha-Proteobacteria and lachnospiraceae;
"Green Cancer" - inflammatory bowel disease has become a common disease in china's digestive tract life, everyone has had stomach pain, tummy tuck, no appetite and other similar experiences, let people really feel the feeling of intestinal discomfort, and hope to have a healthy intestine.
Unfortunately, the incidence of a type of digestive tract disease called inflammatory bowel disease (IBD) is increasing year by year in China, characterized by chronic inflammation of the digestive tract, clinical manifestations of recurrent diarrhea, abdominal pain, stool blood, etc., and with the gradual progress of the disease, IBD patients will appear digestive tract structure and dysfunction, seriously impairing the quality of life of patients and their families, known as "green cancer."
that genetic susceptible genes and a variety of environmental factors together led to the occurrence of IBD, but the specific pathogenesis is still unknown.
In order to gain an in-depth understanding of the pathogenesis of IBD and to find ways to prevent, diagnose and treat IBD, researchers from various countries are exploring it from a number of perspectives, and there have been many studies that have demonstrated the important role of enteric bacterios groups in the development of IBD.
intestinal bacterium is an important factor in maintaining intestinal health, the intestinal bacteribus is designated in the human digestive tract of all bacteria.
we often ignore the presence of gut microbiomes, they actually play a very important role in the health of the body.
intestinal cytosus is a diverse and vibrant bacterial community, and studies have shown that there are more than 1,000 healthy adult intestinal virlocy, with a total population of about 4 x 10E13, equivalent to the number of human cells.
A wide variety of intestinal bacteribus form a large and complex micro-ecosystem with many functions, such as helping digestion, promoting intestinal cell differentiation, protecting the host from pathogens, stimulating and regulating the immune system, and is considered a "hidden organ".
, however, under the influence of internal and external factors, the intestinal bacteria may appear structural and/or dysfunctional, and the disordered intestinal bacteria can induce a variety of diseases.
antimicrobial peptides play an important role in the regulation of the intestinal bacteribus the gut secretes a class of small molecule proteins with antibacterial activity, namely antimicrobial peptides (AMPs), which are mainly secreted by intestinal pans and endotrine cells, a wide variety of broad-spectrum anti-bacterial, fungal and viral activity.
antimicrobial peptides found so far include the defenses family, tissue protease inhibitors, regenerative islet derived protein families, CYT families, lysobacterase families, etc.
clinical testing and laboratory studies have shown that abnormal expression of antimicrobial peptides is closely related to the development of IBD.
, however, there is currently no research on the "personalized" antimicrobial effects of antimicrobial peptides in the body, nor has there been a link between specific microbial groups regulated by antimicrobial peptides and IBD.
, in-depth and detailed exploration of antimicrobial peptide-bacteria-IBD interactions and clarification of their regulatory mechanisms may provide a new path for IBD diagnosis and treatment.
angiogenin inhibits the occurrence of IBD by regulating the steady state of the intestinal bacteriocytes Xu Zhengping/Sheng Jinghao task force uses clinical samples, angiogenin (angiogenin, ANG) gene loss mice and a variety of enteritis models to prove that the intestinal secretion of ANG through Differences regulate the growth of alpha-Proteobacteria and Lachnospiraceae to maintain the micro-ecological balance of the intestines;
team found that a healthy gut secretes the right amount of ANG protein into the intestinal cavity, while IBD patients have only about half the levels of normal people.
to answer whether ANG secretion deficiency was related to the development of IBD, they induced enteritis in mice with a lack of Ang1 gene and in mice with wild types, and found that the symptoms of enteritis in mice with a lack of Ange1 were significantly aggravated.
further, they transferred the feces of mice with a missing Ang1 gene into the intestines of wild mice and found that the latter's enteritis symptoms were significantly worse, suggesting that ANG could affect the IBD process by regulating the gut microbiota.
, using high-flux sequencing techniques, they found significant changes in the gut bacteria of Ang1-missing mice, mainly characterized by an increase in alpha deformation bacteria and a decrease in the gross screw strain.
more significantly, they screened the ANG-regulated strains through bacterial culture and identification techniques, B. diminuta, which is a defective short-wave monocytobacteria (B. diminuta) and S. paucimobilis, which belongs to the anaerostical psips of the hairy glycobacteria, and Blutasia sp.
above results suggest that these differential strains can serve as indicators for clinical diagnosis of IBD, and that the two mycobacterial strains have the potential to prevent or treat IBD and may be developed as probiotic preparations for clinical use in the future.
, the team revealed how ANG regulates the gut bacteria.
Through in-body antibacterial experiments and scanning electroscopes and other means, they found that ANG can selectively combine and effectively kill alpha deformation bacteria, and further in-body bacterial culture experiments show that there is mutual inhibition between alpha deformation bacteria and hairy bacteria, which explains why ANG can inhibit the alpha deformation bacteria at the same time can promote the growth of the hairy bacteria.
, the team explored the feasibility of ANG regulating the intestinal bacteria and reducing IBD.
by recombining the ANG1 protein by supplementing the digestive tract of mice with Ange1 deficiency, the disorder of the intestinal bacteria in mice was effectively responded to, and the symptoms of enteritis were significantly reduced.
this provides experimental evidence for ANG's clinical transformation application.
, Professor Xu Zhengping and Professor Sheng Jinghao of Zhejiang University School of Medicine, Liangyu Laboratory (Systems Medicine and Precision Diagnosis and Treatment In Zhejiang Province) are co-authors of the paper.
, a doctoral student at Zhejiang University School of Medicine, Bai Rong pan, a postdoctoral student, and Zhou Wei, chief physician of general surgery at Yifu Hospital, affiliated with Zhejiang University School of Medicine, co-authored the paper.
research results were funded by the National Natural Science Foundation of China and the Natural Science Foundation of Zhejiang Province.
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