 Home > Biochemistry News > Plant Extracts News > Global Identification of Small RNA Targets in Plants by Sequencing Sliced Ends of Messenger RNAs

Global Identification of Small RNA Targets in Plants by Sequencing Sliced Ends of Messenger RNAs

 Last Update: 2020-11-21
 Source: Internet
 Author: User

Tags

plants

small size

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Small RNAs (microRNAs and other classes of endogenous small interfering RNAs) play important roles in a wide variety of biological processes. However, integration of small RNAs in diverse biological networks relies on the confirmation of their RNA targets. In plants, miRNAs negatively regulate mRNA targets by guiding a cleavage in the complementary site that leaves a 3′ polyadenylated RNA possessing monophosphate at its 5′ end. This chapter describes a detailed step-by-step protocol for cloning such sliced 3′ products in order to identify small RNA targets. Using this protocol, we have identified more than 150 small RNA targets in rice; some are conserved and others are non-conserved targets for rice small RNAs.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Monobenzone CAS 103-16-2 White Crystal Powder USP/BP Standard

CAS No.: 103-16-2

Cosmetics Grade

$1-1.2/KG EXW

Carob Extract

CAS No.: 84961-45-5

pharmaceutical grade/food grade

$10-13/KG FOB

Premium ingredient Panax Ginseng Extract 80%

CAS No.: 50647-08-0

Food Grade

$25-30/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.