-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Prime Editor (PE ) is a new type of genome editing tool that can achieve arbitrary base substitution and precise deletion and insertion of small fragments at the target site of the genome.
Recently, Caixia Gao's research group and collaborators from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences published research results, and successfully established a more efficient and widely applicable new guided editing system ePPE ( Engineered Plant Prime Editor ) in plants
In summary, the researchers successfully developed a new guided editing system ePPE that can achieve efficient editing in plants by focusing on the modification of the protein components of PPE , and proved that the combination of ePPE and the existing pegRNA optimization strategy can further improve the editing efficiency.
Figure: The establishment and application of ePPE , an efficient new bootstrap editor for plants .
ab , Protoplast reporter system for screening candidate plant guided editing systems
c , Comparison of the editing efficiency of the guide editor constructed based on the deletion of the RNase H domain and the fusion of the NC protein with the PPE .
d, Schematic diagram of ePPE
f , Comparison of editing efficiency of ePPE combined with reported pegRNA optimization strategies
g , Phenotype of ALS-W548M rice mutant under different herbicide treatments
