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Functional Analysis by Protein Biochemistry

 Last Update: 2020-11-09
 Source: Internet
 Author: User

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structural functional analysis

what scientists use

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To date a number of cereal genomes are fully sequenced and more are near completion. The information within these genomes will be of most use to scientists when every gene has been functionally characterized leading to the complete annotation of these genomes. This chapter describes how functional characterization of plant proteins can be achieved via in vitro or in vivo methods. The first section of this chapter describes the use of
Escherichia coli
as a host for expression of plant genes, followed by purification and in vitro characterization of the resultant enzyme. The second section of this chapter details the methods involved in transient gene expression in
Zea mays
leaf protoplasts for in vivo functional characterization of protein localization.

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