Four Seasons of Cynophenolic Acid

Extraction source: This product is the phenolic acid extracted from the leaves of holly plant holly llex chinensis Sims, with a total phenolic acid content of 80%main ingredients: original tea acid Protocatechuic acid, content: 40%p style": "text-align: center;" img alt""four-season physioic acid" src"/uploads/allimg/1701/2-1F12015191G21.jpg" Height: 134px;the original tea aldehyde protocatecaldehydecoffee acid Caffeic acidwinter sapin Ilexosidecontent: 40%the product is a light brown to brown yellow powder, soluble in methanol, ethanol, hot water"Identification" take this product with methanol to make 0.5% solution for test ingeTake standard raw tea acid, raw tea aldehyde, coffee acid and other methanol to make each containing 1mg / ml solution for controlTake four seasons green standard control of the medicinal herbs 1.0g with methanol 30 ml, ultrasound extraction for 30 minutes, the filter concentrated to 1 ml of control fluidTake the above three liquid s10 sl, respectively, on the same silicone GF254 plate, to chloroform-methanol-to-toicacid (7:1:0.2) to expand, UV lamp 254nm inspection, and then spray 1% vanilla aldehyde sulfuric acid solution, hot windThe corresponding position of the standard speckle appears the same color fluorescent spots and visible spots, compared to the standard medicinal spots, the corresponding rate of 60%Dry weightlessness: No more than 4.0% (Appendix IX.G)Burning Residue: No more than 0.5% (Appendix IX.J)Heavy Metals: No more than 10ppm (Appendix IX.E)"Content Determination" according to high-efficiency liquid chromatography (Appendix VI.D) determination chromatography condition C18 column mobile phase: acetylene 1% acetic acid (5:95) detection wavelength: 260nm the number of theory boards is 3000 control fluid: Take the original catechinacid label with methanol -1% ice acetic acid (70:30) mixture to make a 50Ug/ml solution backup for test fluid: Take this product with methanol -1% ice acetic acid (70:30) mixture to make 0.1mg/ml solution backup the determination: take the above two-liquid 10 sl injection liquid chromatography, and calculate the total phenolic acid content function siphons of coronary arteries, anti-inflammatory, anti-tumor storage is cool, dry and light-proof